Zamora-Mendoza, R.R.Zamora-MendozaRosas-Vargas, H.H.Rosas-VargasRamos-Cervantes, M. T.M. T.Ramos-CervantesGarcía-Zúñiga, P.P.García-ZúñigaPérez-Lorenzana, H.H.Pérez-LorenzanaMendoza-Lorenzo, PatriciaPatriciaMendoza-LorenzoMiliar-García, A.A.Miliar-GarcíaLara-Padilla, E.E.Lara-PadillaEstrada Mena, Francisco JavierFrancisco JavierEstrada MenaCeballos, GuillermoGuillermoCeballosRodríguez, AlonsoAlonsoRodríguezVillarreal, Francisco J.Francisco J.VillarrealRamírez-Sánchez, IsraelIsraelRamírez-Sánchez2023-01-192023-01-192017https://scripta.up.edu.mx/handle/20.500.12552/224810.1038/ijo.2017.274Background/Objectives: We aimed to evaluate mitochondrial biogenesis (MB), structure, metabolism and dysfunction in abdominal adipose tissue from male pediatric patients with obesity. Subjects/Methods: Samples were collected from five children with obesity (percentile ⩾95) and five eutrophic boys (percentile ⩾5/⩽85) (8–12 years old) following parental informed consent. We analyzed the expression of key genes involved in MB (sirtuin-1 (SIRT1), peroxisome proliferator-activated receptor-γ (PPARγ), PPARγ coactivator-1α (PGC1α), nuclear respiratory factors 1 and 2 (NRF1, NRF2) and mitochondrial transcription factor A (TFAM) and surrogates for mitochondrial function/structure/metabolism (porin, TOMM20, complex I and V, UCP1, UCP2, SIRT3, SOD2) by western blot. Citrate synthase (CS), complex I (CI) activity, adenosine triphosphate (ATP) levels, mitochondrial DNA (mtDNA) content and oxidative stress end points were also determined.Resource Types::text::journal::journal article