Embryonary mouse cardiac fibroblast isolation

Garate-Carrillo, Alejandra; Ramírez-Sánchez, Israel

doi:10.1007/978-1-4939-7714-7_7

Embryonary mouse cardiac fibroblast isolation

Journal

Mouse Embryogenesis

Methods in Molecular Biology

ISSN

1064-3745

1940-6029

Date Issued

2018

Author(s)

Garate-Carrillo, Alejandra

Ramírez-Sánchez, Israel

Facultad de Ciencias de la Salud - CampCM

Type

text::book::book part

DOI

10.1007/978-1-4939-7714-7_7

URL

https://scripta.up.edu.mx/handle/20.500.12552/2250

Abstract

Mouse cardiac fibroblasts have been widely used as an in vitro model for studying fundamental biological processes and mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Cardiac FBs are relatively easy to culture in a dish and can be manipulated using molecular and pharmacological tools. Because FBs rapidly decrease cell cycle division and proliferative rate after birth, they are prone to phenotypic changes and senescence in cell culture soon after a few passages. Therefore, primary cultures of differentiated fibroblasts from embryos are more desirable. Below we will describe a method that provides good cell yield and viability of E16 CD-1 mouse embryonic cardiac fibroblasts in primary cultures. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.