Comparison of TGF-beta 1 and TGF-beta 2 in serum and aqueous humor levels in diabetic patients with and without macular edema
Journal
Investigative Ophthalmology & Visual Science
ISSN
1552-5783
Date Issued
2020
Author(s)
Zepeda-Palacio, Claudia
Antonio-Aguirre, Bani
Pita-Ortiz, Ingrid Y.
Rosado-Lozoya, Johanna S.
Robles Contreras, Atzin
Hernández, Ángeles
Type
text::journal::journal article
Abstract
Purpose : Cytokine-mediated pericyte loss is a cornerstone in the development of diabetic macular edema (DME) among non-proliferative diabetic retinopathy (NPDR). In vitro, TGF-β increased expression leads to pericytal apoptosis and macular edema (through the breakdown in the blood-retinal barrier). To date, there are no reports on TGF-b expression in vivo. Here, we assessed the relationship between TGF-β1 and -β2 expression levels in plasma and aqueous humor (AH) of patients with NPDR with and without DME. Methods : We performed a case-control study of 53 patients clinically diagnosed with NPDR. Cases had a recent diagnosis of DME and were starting an antiangiogenic treatment. Controls were patients without EMD undergoing phacoemulsification for senile cataracts. We measured visual acuity, LOCS-III and RDNP staging, macular OCT, HbA1c, and collected serum and aqueous humor samples. We measured the concentration of TGF-β1 and -β2 by cytometric-bead assays. We performed a Student's T-test and linear regressions to adjust our results with SAS V 9.4. © Investigative Ophthalmology & Visual Science