rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism

Bobadilla-del-Valle, Miriam; Ponce-de-León, Alfredo; Arenas-Huertero, Catalina; Vargas Alarcón, Gilberto; Kato-Maeda, Midori; Small, Peter M.; Couary, Patricia; Ruiz-Palacios, Guillermo M.; Sifuentes-Osornio, José

rpoB gene mutations in rifampin-resistant Mycobacterium tuberculosis identified by polymerase chain reaction single-stranded conformational polymorphism

Journal

Emerging Infectious Diseases

ISSN

1080-6059

Date Issued

2001

Author(s)

Bobadilla-del-Valle, Miriam

Ponce-de-León, Alfredo

Arenas-Huertero, Catalina

Vargas Alarcón, Gilberto

Facultad de Ciencias de la Salud - CampCM

Kato-Maeda, Midori

Small, Peter M.

Couary, Patricia

Ruiz-Palacios, Guillermo M.

Sifuentes-Osornio, José

Type

text::journal::journal article

URL

https://scripta.up.edu.mx/handle/20.500.12552/2548

Abstract

The use of polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) to study rpoB gene mutations in rifampin-resistant (RIFr) Mycobacterium tuberculosis has yielded contradictory results. To determine the sensitivity of this method, we analyzed 35 RIFr strains and 11 rifampin-susceptible (RIFs) strains, using the DNA sequencing of the core region of rpoB for comparison. Of the RIFr, 24 had a PCR-SSCP pattern identical to that of H37Rv; the other 11 had four different patterns. The 11 RIFs had PCR-SSCP patterns identical to that of H37Rv. The sensitivity of the assay was 31.4%; its specificity was 100%. We observed a strong correlation between the degree of resistance and the type of mutation.