Efecto del tratamiento con microondas e inhibidores en el oscurecimiento enzimático y carotenoides de plátano macho (Musa paradisiaca L.)

The object of this study is to evaluate the effect of heat treatment by microwaves and the use of some chemical inhibitors on the enzymatic activity of the poliphcnoloxidase of plantain (Musa paradisiaca L.). During the experimental phase the IN VITRO effect will be determined through specific enzymatic kinetics. The enzyme will be extracted and purified from the pulp. Based on the obtained results the best inhibition conditions will be selected and will be applied directly to plantain slices simulating the industrial treatment. Plantain represents the principal source of energy in certain subsistence diets. It is a good source of potassium and Β-carotene and a respectable source of fiber and vitamin C. Β-carotene, apart from being the carotenoid of greater vitaminic activity (it consists of two molecules of vitamin A), has antioxidant properties; this is the reason why it is important to extract, quantify and evaluate the effect heat treatment by microwaves, frying and the use of inhibitors have on it. HPLC will be used for this effect. The search for new alternatives to avoid the browning of plantain arises from the need to substitute sulfites. In 1985, it was concluded that they were safe for most people, however they represent an unpredictable and severe risk for asthmatic people and for those who are sensitive to these preservatives. It must be said that any person may develop sulfite sensitivity at any stage of his life. The physiological response brought upon by sulfites may go from breathing difficulty and rash, to breathing and heart failure. Since one of the alternative uses of the production plantain surplus, is as chips commercialized as snacks, they are consumed mainly by children; this must be considered as a limitation for the use of additives according to the joint established regulations of FAO and CODEX ALIMENTARIUS.

Introducción -- Justificación -- Generalidades de la materia prima, motivo de la investigación -- Antecedentes -- Polifenoloxidasa -- Sulfitos -- Tratamientos alternativos -- Objetivo general -- Objetivos específicos