Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model

González-Pacheco, Héctor; Méndez-Domínguez, Aurelio; Hernández Gutiérrez, Salomón; López-Marure, Rebeca; Vazquez-Mellado, Maria J.; Aguilar, Cecilia; Rocha-Zavaleta, Leticia

doi:10.1155/2014/187071

Pre-Conditioning with CDP-Choline Attenuates Oxidative Stress-Induced Cardiac Myocyte Death in a Hypoxia/Reperfusion Model

Journal

The Scientific World Journal

ISSN

2356-6140

1537-744X

Date Issued

2014

Author(s)

González-Pacheco, Héctor

Méndez-Domínguez, Aurelio

López-Marure, Rebeca

Vazquez-Mellado, Maria J.

Aguilar, Cecilia

Rocha-Zavaleta, Leticia

Type

Resource Types::text::journal::journal article

DOI

10.1155/2014/187071

URL

https://scripta.up.edu.mx/handle/20.500.12552/2353

Abstract

Background: CDP-choline is a key intermediate in the biosynthesis of phosphatidylcholine, which is an essential component of cellular membranes, and a cell signalling mediator. CDP-choline has been used for the treatment of cerebral ischaemia, showing beneficial effects. However, its potential benefit for the treatment of myocardial ischaemia has not been explored yet. Aim: In the present work, we aimed to evaluate the potential use of CDP-choline as a cardioprotector in an in vitro model of ischaemia/reperfusion injury. Methods: Neonatal rat cardiac myocytes were isolated and subjected to hypoxia/reperfusion using the coverslip hypoxia model. To evaluate the effect of CDP-choline on oxidative stress-induced reperfusion injury, the cells were incubated with H₂O₂ during reperfusion. The effect of CDP-choline pre- and postconditioning was evaluated using the cell viability MTT assay, and the proportion of apoptotic and necrotic cells was analyzed using the Annexin V determination by flow cytometry.