Effect of Vitamin E on Diabetic Nephropathy in Streptozotocin-Induced Diabetic Rats
Journal
International Journal of Molecular Sciences
ISSN
1422-0067
Publisher
MDPI AG
Date Issued
2025-02-13
Author(s)
Segura Cobos, David
Díaz Salgado, Esperanza Enedina
Amato, Dante
Cardoso García, Sinaí Ernesto
Tomás Ernesto Villamar Duque
Heredia Antúnez, Anayantzin Paulina
Del Valle-Mondragón, Leonardo
Magos Guerrero, Gil Alfonso
Guzmán Hernández, Elizabeth Alejandrina
Type
text::journal::journal article
Abstract
Diabetic nephropathy (DN) is a serious complication of diabetes mellitus; oxidative stress plays a key role in the pathogenesis of DN. The objective of this study was to evaluate the antioxidant effect of vitamin E on diabetic nephropathy. A control group and three groups of rats with streptozotocin-induced diabetes mellitus (untreated diabetic rats and diabetic rats treated with vitamin E 250 and 500 mg/kg) were studied. After 4 weeks of treatment, the kidneys were removed under anesthesia with sodium pentobarbital. The kidneys were weighed, the AT1 and AT2 receptor expression was measured by Western blot, and the activities of glutathione peroxidase, catalase, and superoxide dismutase were determined in the renal cortex. Rats with diabetes mellitus had hyperglycemia, increased food and water consumption, and higher urinary volume than control rats. In diabetic rats (DM), kidney hypertrophy was observed and measured by kidney weight, protein/DNA ratio in the renal cortex, and proximal tubular cell area; proteinuria and reduced creatinine clearance were observed. AT1 and AT2 receptor expression in the kidney cortex of DM rats increased significantly compared to normoglycemic rats; antioxidant enzyme activities were decreased; treatment with vitamin E reversed kidney hypertrophy and reduced proteinuria; reduction in expression of AT1 and AT2 receptors was associated with increased antioxidant activity. Thus, treatment with vitamin E slows the progress of DN. ©The authors ©International Journal of Molecular Sciences ©MDPI.
License
Acceso Abierto
How to cite
Segura Cobos, D., Díaz Salgado, E. E., Amato, D., Cardoso García, S. E., Villamar Duque, T. E., Antúnez, A. P. H., del Valle Mondragón, L., Magos Guerrero, G. A., & Guzmán Hernández, E. A. (2025). Effect of Vitamin E on Diabetic Nephropathy in Streptozotocin-Induced Diabetic Rats. International Journal of Molecular Sciences, 26(4), 1597. https://doi.org/10.3390/ijms26041597