Pharmacokinetics of Sirolimus in a Novel Liposome Delivery System in Selected Ocular Tissues and Plasma Following a Single Subconjunctival Injection in Dutch Belted Rabbits
Journal
Journal of Ocular Pharmacology and Therapeutics
ISSN
1080-7683
1557-7732
Date Issued
2022
Author(s)
Brooks, Dennis
Linares-Alba, Monica Anayatzin
Garcia-Santisteban, Rodrigo
Xie, Enli
Gum, Glenwood
Manza, Linda L.
Servitje-Azcarraga, Lucila
Santisteban, Diego G.
García-Sánchez, Gustavo Adolfo
Type
text::journal::journal article
Abstract
Purpose: To determine the pharmacokinetics of a proprietary liposomal sirolimus (LS) formulation in ocular tissues and plasma following a single subconjunctival (SCJ) injection in Dutch belted rabbits (DBR). Analytical methods for detection of LS in plasma, aqueous humor (AH), vitreous humor (VH), retina, combined retina/choroid/retinal pigment epithelium, sclera, and iris/ciliary body were developed to examine samples. Methods: Thirty male DBR were subconjunctivally injected in both eyes with 0.1 mL of LS of 1,000 μg/mL. At selected times post-injection, ocular tissues and whole blood samples were obtained. Sirolimus concentrations were measured using liquid chromatography/tandem mass spectrometry.
Subjects