Dysregulation of mitochondrial function and biogenesis modulators in adipose tissue of obese children

Zamora-Mendoza, R.; Rosas-Vargas, H.; Ramos-Cervantes, M. T.; García-Zúñiga, P.; Pérez-Lorenzana, H.; Mendoza-Lorenzo, Patricia; Miliar-García, A.; Lara-Padilla, E.; Estrada Mena, Francisco Javier; Ceballos, Guillermo; Rodríguez, Alonso; Villarreal, Francisco J.; Ramírez-Sánchez, Israel

doi:10.1038/ijo.2017.274

Dysregulation of mitochondrial function and biogenesis modulators in adipose tissue of obese children

Journal

International Journal of Obesity

ISSN

0307-0565

1476-5497

Date Issued

2017

Author(s)

Zamora-Mendoza, R.

Rosas-Vargas, H.

Ramos-Cervantes, M. T.

García-Zúñiga, P.

Pérez-Lorenzana, H.

Mendoza-Lorenzo, Patricia

Miliar-García, A.

Lara-Padilla, E.

Ceballos, Guillermo

Rodríguez, Alonso

Villarreal, Francisco J.

Ramírez-Sánchez, Israel

Type

Resource Types::text::journal::journal article

DOI

10.1038/ijo.2017.274

URL

https://scripta.up.edu.mx/handle/20.500.12552/2248

Abstract

Background/Objectives: We aimed to evaluate mitochondrial biogenesis (MB), structure, metabolism and dysfunction in abdominal adipose tissue from male pediatric patients with obesity. Subjects/Methods: Samples were collected from five children with obesity (percentile ⩾95) and five eutrophic boys (percentile ⩾5/⩽85) (8–12 years old) following parental informed consent. We analyzed the expression of key genes involved in MB (sirtuin-1 (SIRT1), peroxisome proliferator-activated receptor-γ (PPARγ), PPARγ coactivator-1α (PGC1α), nuclear respiratory factors 1 and 2 (NRF1, NRF2) and mitochondrial transcription factor A (TFAM) and surrogates for mitochondrial function/structure/metabolism (porin, TOMM20, complex I and V, UCP1, UCP2, SIRT3, SOD2) by western blot. Citrate synthase (CS), complex I (CI) activity, adenosine triphosphate (ATP) levels, mitochondrial DNA (mtDNA) content and oxidative stress end points were also determined.