The sarcoglycan–sarcospan complex localization in mouse retina is independent from dystrophins

Fort, Patrice; Estrada Mena, Francisco Javier; Bordais, Agnès; Mornet, Dominique; Sahel, Jose-Alain; Picaud, Serge; Rosas Vargas, Haydeé; Coral-Vázquez, Ramón; Rendon, Álvaro

doi:10.1016/j.neures.2005.05.007

The sarcoglycan–sarcospan complex localization in mouse retina is independent from dystrophins

Journal

Neuroscience Research

ISSN

0168-0102

Date Issued

2005

Author(s)

Fort, Patrice

Estrada Mena, Francisco Javier

Facultad de Ciencias de la Salud - CampCM

Bordais, Agnès

Mornet, Dominique

Sahel, Jose-Alain

Picaud, Serge

Rosas Vargas, Haydeé

Coral-Vázquez, Ramón

Rendon, Álvaro

Type

text::journal::journal article

DOI

10.1016/j.neures.2005.05.007

URL

https://scripta.up.edu.mx/handle/20.500.12552/2510

Abstract

The sarcoglycan–sarcospan (SG–SSPN) complex is part of the dystrophin–glycoprotein complex that has been extensively characterized in muscle. To establish the framework for functional studies of sarcoglycans in retina here, we quantified sarcoglycans mRNA levels with real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and performed immunohistochemistry to determine their cellular and subcellular distribution. We showed that the β-, δ-, γ-, ɛ-sarcoglycans and sarcospan are expressed in mouse retina. They are localized predominantly in the outer and the inner limiting membranes, probably in the Müller cells and also in the ganglion cells axons where the expression of dystrophins have never been reported. We also investigated the status of the sarcoglycans in the retina of mdx3cv mutant mice for all Duchene Muscular Dystrophy (DMD) gene products. The absence of dystrophin did not produce any change in the sarcoglycan–sarcospan components expression and distribution.